Enzymatic Production of l-Alanine by Pseudomonas dacunhae
نویسندگان
چکیده
منابع مشابه
Production of L-DOPA(3,4-dihydroxyphenyl-L-alanine) from benzene by using a hybrid pathway.
As an alternative approach to the production of L-DOPA from a cheap raw material, we constructed a hybrid pathway consisting of toluene dioxygenase, toluene cis-glycol dehydrogenase, and tyrosine phenol-lyase. In this pathway, catechol is formed from benzene through the sequential action of toluene dioxygenase and toluene cis-glycol dehydrogenase, and L-DOPA is synthesized from the resulting ca...
متن کاملAffinity labeling of alanine aminotransferase by 3-chloro-L-alanine.
The pyridoxal form of alanine aminotransferase from pig heart catalyzes the a,/? elimination reaction with 3chloro-L-alanine as the substrate to form equimolar amounts of pyruvate, ammonia, and chloride. The maximum rate of the a,/3 elimination reaction was 2.5 pmol/min/mg at pH 7.0 (25”(Z), approximately 0.5% that of the transamination reaction between L-alanine and 2-oxoglutarate. Time-depend...
متن کاملMedium Optimization for Enzymatic Production of L-Cysteine by Pseudomonas sp. Zjwp-14 Using Response Surface Methodology
Response surface methodology was applied to optimize medium constituents for enzymatic production of L-cysteine from DL-2-amino-D-thiazoline-4-carboxylic acid (DL-ATC) by a novel Pseudomonas sp. Zjwp-14. With the Plackett-Burman design experiment, glycerol, DL-ATC, yeast extract, and pH were found to be the most powerful factors among the eight tested variables that influence intracellular enzy...
متن کاملImproved Production of Rhamnolipids by a Pseudomonas aeruginosa Mutant
A Pseudomonas aeruginosa mutant derived by random mutagenesis with N-methyl-N¢-nitro-N-nitrosoguanidine, producing high levels of the rhamnolipid biosurfactants was selected on Siegmund-Wagner (SW) plates. The mutant designated P. aeruginosa PTCC1637 produces rhamnolipids at concentrations 10 time more than parent strain. NMR analysis and surface tension measurement showed that the biosurfactan...
متن کاملTechnique for Improved Production of 3,4 Dihydroxy Phenyl L-alanine by Aspergillus Oryzae
In the present study, mould mycelium of Aspergillus oryzae was used for biochemical conversion of L-tyrosine to L-DOPA because tyrosinase is an intracellular enzyme. The mutant Aspergillus oryzae IIB-6 was found to yield 3.72 fold higher production of L-DOPA than the parental strain. The comparison of kinetic parameters was also done which showed the greater ability of mutant to yield L-DOPA. W...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1965
ISSN: 0003-6919
DOI: 10.1128/aem.13.5.638-645.1965